Journal: Scientific Reports
Article Title: Novel histological observation method using the scattered light of unstained collagen sections
doi: 10.1038/s41598-026-38504-9
Figure Lengend Snippet: Scattering angle-resolved bioimaging. ( A ) Schematic of the experimental setup. The checker pattern consists of illuminated white patches and non-illuminated black patches. By translating the pattern, two types of images—illuminated and non-illuminated regions—can be acquired. ( B ) Transmitted light microscope (Olympus BX53) equipped with a halogen lamp, bandpass filter, high-frequency checker pattern filter, and a camera (GS3-U3-60QS6, FLIR Systems). ( C) The high-frequency checker pattern filter consists of a photomask with four binary checker patterns, positioned between the field diaphragm and the condenser lens.
Article Snippet: The imaging system consisted of a transmitted light microscope (Olympus BX53 and BX63) equipped with a halogen lamp, a custom-designed high-frequency checkerboard photomask (NIPPON FILCON CO., LTD), and a camera (GS3-U3-60QS6, FLIR Systems).
Techniques: Light Microscopy